LOCUS       40924_7086        6500 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBS 35S attR-YFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6500)
  AUTHORS   Subramanian C, Woo J, Cai X, Xu X, Servick S, Johnson CH, Nebenfuhr 
            A, von Arnim AG.
  TITLE     A suite of tools and application notes for in vivo protein 
            interaction assays using bioluminescence resonance energy transfer 
            (BRET)
  JOURNAL   Plant J. 48 (1), 138-152 (2006)
  PUBMED    16925598
REFERENCE   2  (bases 1 to 6500)
  AUTHORS   von Arnim AG, Subramanian C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2005) BCMB, University of Tennessee Knoxville, 
            1406 Circle Drive, Hesler Biology Bldg 618, Knoxville, TN 37996, USA
REFERENCE   3  (bases 1 to 6500)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6500)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2006"; volume: "48"; issue: "1"; pages: "138-152"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-MAR-2005) BCMB, University of Tennessee Knoxville, 1406 Circle 
            Drive, Hesler Biology Bldg 618, Knoxville, TN 37996, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6500
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(6..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     669..685
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        789..1460
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     regulatory      1460..1602
                     /note="translational enhancer; 5' UTR from tobacco etch
                     virus"
                     /regulatory_class="enhancer"
     protein_bind    1608..1732
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1757..1787
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1841..2497
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             2842..3144
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(3188..3312)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3314..4030
                     /label=EYFP
                     /note="enhanced YFP"
     regulatory      4043..4253
                     /label=35S cauliflower mosaic virus terminator
                     /note="35S cauliflower mosaic virus terminator"
                     /regulatory_class="terminator"
     promoter        complement(4315..4333)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4354..4370)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4378..4394)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4402..4432)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4447..4468)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4756..5344)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5518..6375)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6376..6480)
                     /label=AmpR promoter
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gcccccctcg aggtcgacgg tatcgataag cttgatatcg aattcctgca ggtcaacatg
      721 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
      781 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
      841 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
      901 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
      961 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1021 aagcaagtgg attgatgtga taacatggtg gagcacgaca cacttgtcta ctccaaaaat
     1081 atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata
     1141 tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg
     1201 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa
     1261 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
     1321 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac
     1381 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt
     1441 tcatttcatt tggagaggac ctcgagaatt ctcaacacaa catatacaaa acaaacgaat
     1501 ctcaagcaat caagcattct acttctattg cagcaattta aatcatttct tttaaagcaa
     1561 aagcaatttt ctgaaaattt tcaccattta cgaacgatag gcctaggaca agtttgtaca
     1621 aaaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc
     1681 ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggccgc
     1741 attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt ggattttgag
     1801 ttaggatccg tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
     1861 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
     1921 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
     1981 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
     2041 gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag
     2101 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
     2161 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
     2221 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc
     2281 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
     2341 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct
     2401 ggcgattcag gttcatcatg ccgtctgtga tggcttccat gtcggcagaa tgcttaatga
     2461 attacaacag tactgcgatg agtggcaggg cggggcgtaa acgcgtggat ccggcttact
     2521 aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac
     2581 tgatatgtat acccgaagta tgtcaaaaag aggtgtgcta tgaagcagcg tattacagtg
     2641 acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc
     2701 tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg
     2761 aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg
     2821 acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt
     2881 tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg acggatggtg
     2941 atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg
     3001 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc
     3061 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
     3121 attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca
     3181 ggtcgaccat agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg
     3241 caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt cagctttctt
     3301 gtacaaagtg gtcatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     3361 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     3421 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     3481 gccctggccc accctcgtga ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc
     3541 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     3601 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     3661 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     3721 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     3781 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     3841 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     3901 gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg
     3961 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     4021 gctgtacaag agatctatct agagtccgca aaaatcacca gtctctctct acaaatctat
     4081 ctctctctat ttttctccag aataatgtgt gagtagttcc cagataaggg aattagggtt
     4141 cttatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt
     4201 gtaaaatact tctatcaata aaatttctaa ttcctaaaac caaaatccag tgacctgcag
     4261 cccgggggat ccactagagc ggccgccacc gcggtggagc tccagctttt gttcccttta
     4321 gtgagggtta atttcgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     4381 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     4441 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     4501 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     4561 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     4621 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     4681 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     4741 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     4801 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     4861 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     4921 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     4981 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     5041 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     5101 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     5161 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     5221 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     5281 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     5341 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     5401 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     5461 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     5521 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     5581 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     5641 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     5701 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     5761 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     5821 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     5881 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     5941 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     6001 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     6061 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     6121 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     6181 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     6241 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     6301 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     6361 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     6421 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     6481 cacatttccc cgaaaagtgc
//
